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Samples which absorb short-wave UV at 254 nm are detected due to fluorescence quenching.
In addition, F254s is highly stable in acidic solvent systems. Both indicators fluorescence in UV light at an excitation wavelength of 254 nm.
For UV detection of colorless substances, plates with two kinds of fluorescent indicator are available: green fluorescing F254 (manganese activated zinc silicate) or blue fluorescing F254s (magnesium wolframate).
Pack of 10, glass backed TLC silica gel plate with excellent reproducibility; mainly used for qualitative and quantitative analysis of laboratory samples
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These plates have neutral silica (pH 6-7) instead of the more common acidic silica (pH=5) for thin layer chromatography silica plates,
Classic thin layer chromatography silica plates, GF254 type (fluorescence background at 254nm), 60 angstroms pore size. Aluminum backed-easily cut by a pair of scissors.
TLC Chromatography Plate : Thin layer chromatography silica gel plate can be directly used for qualitative or quantitative analysis of various types of organic substances
Good Separation Effect : Short separation time, high sensitivity, clear spots, non diffusing
Glass Backed TLC Classic Silica Plate : The main component is SiO2 · nH2O, and the coated silicone GF254 contains calcined gypsum and fluorescent agent
A test run separating dimethyl maleate and dimethyl fumerate has been performed and shown in photos.
50mg of each compound (total mass 100mg), dissolved in a small amount mixture of hexane, acetone and dichloromethane was spotted onto the plate. The plate was eluted twice with hexane:acetone=15:1, giving a very good separation. While this is expected, this also indicated that depending on how far away each component band is on TLC, one can load material amounts of 100mg and more onto the plate since in the example the plates have not quite reached full capacity.
Size: 7.9 x 7.9 Inch, pack of 10L; layer thickness: 1000 µm; particle size: 10 - 40 µm; PH: 6.2 - 6.8; pore size: 60 Å
Silica Precision: crafted using unaltered silica gel and a gypsum binder to ensure superior separation accuracy, heightened sensitivity, and a diverse range of chromogenic agent options
Set of 10: glass-backed TLC silica gel plates offering outstanding consistency; primarily utilized for qualitative and quantitative analysis of lab specimens; well-suited for applications with modest sample quantities
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